sgrna oligonucleotide library (Twist Bioscience)
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Twist Bioscience
sgrna oligonucleotide library
Sgrna Oligonucleotide Library, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+oligonucleotide+library/library+sgrna/bio_rxiv__64898__2026__03__07__710282-324-1-7
Average 86 stars, based on 1 article reviews
Sgrna Oligonucleotide Library, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+oligonucleotide+library/library+sgrna/bio_rxiv__64898__2026__03__07__710282-324-1-7
Average 86 stars, based on 1 article reviews
sgrna oligonucleotide library - by Bioz Stars,
2026-09
86/100 stars
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1) Product Images from "Reprogramming of neuronal genome function and phenotype by astrocytes"
Article Title: Reprogramming of neuronal genome function and phenotype by astrocytes
Journal: bioRxiv
doi: 10.64898/2026.03.07.710282
Figure Legend Snippet: (A) Volcano plot showing the changes of n=222 ArN TF genes selected for Perturb-seq between N and N+A conditions, average of 4 time points. (B) Table showing statistics of sgRNA library design and data quality metrics for CRISPRi and CRISPRa pRE screens. (C) Scatterplot of showing the numbers of CRISPRi vs CRISPRa pRE targets per ArN TF gene, with genes that are up-regulated by astrocytes at the mRNA level colored in red, and genes down-regulated by astrocytes colored by blue. (D) Barplot showing that the dCas9-p300 cell line activates GRIN2A and MYOD genes by sgRNAs targeted at promoters and a distal regulatory element in differentiated neurons. (E) Histogram showing the distributions of base-pair distance between functional RE and target genes identified in the CRISPRi and CRISPRa pRE screens, respectively. (F) Barplots showing the distribution of # of target genes assigned to a functional RE, and # of functional REs assigned to a target gene, respectively. (G) Barplot showing the effects of CRISPRi on two different functional REs of the TF gene SIX3. The statistical test was done by one-way ANOVA followed by Tukey’s multiple comparisons test. **: adjusted p<0.01; ***: adjusted p<0.001. (H) Schematics showing a high-level overview of the single-cell analysis pipeline of the Perturb-seq screens, highlighting the methods used.
Techniques Used: Functional Assay, Single-cell Analysis
Figure Legend Snippet: (A) Stacked bar plots showing the numbers of targeted annotated primary TSSs, alternative TSSs, and unannotated TSSs based on the RNA-seq data generated in this study, in the CRISPRi/a promoter Perturb-seq experiments, respectively. (B) Table showing statistics of sgRNA library design and data quality metrics for CRISPRi and CRISPRa promoter screens. (C) Violin plot showing the number of cells per target region in the CRISPRa promoter Perturb-seq experiment, highlighting the MYC promoter as an outlier. (D) Violin plot showing the log2FC of the direct target genes in the CRISPRi and CRISPRa promoter Perturb-seq experiments, respectively, separated by positive controls (regardless of significance), TF genes with significant alteration in expression level (Sig. perturb.; p < 0.05), and TF genes with non-significant alteration (N.S. perturb.; p >= 0.05). (E) Jitter plot showing the basal expression levels of CRISPRi promoter target genes that were successfully (‘success’) or unsuccessfully (‘fail’) repressed (F) Bar plot showing the numbers of total global perturbation-gene links discovered by the 4 Perturb-seq experiments. Only those from the positive controls, statistically significant promoters (for the promoter experiments), and fREs (for the pRE experiments) are shown. (G) Jitter plot showing the numbers of global perturbation-gene links per perturbation. Only those from the positive controls, statistically significant promoters (for the promoter experiments), and fREs (for the pRE experiments) are shown. Top perturbations are labeled by its local or direct target gene, for fRE and promoter, respectively. Red bolded are positive controls.
Techniques Used: RNA Sequencing, Generated, Expressing, Labeling
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Synthesized:Article Title: Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes in Corynebacterium glutamicum . Article Snippet: .. The final Article Title: Reprogramming of neuronal genome function and phenotype by astrocytes Article Snippet: The annealed duplex was diluted 1:200 and cloned into Esp3I-digested (NEB, R0734) and gel-purified (Qiagen, 28706) lentiviral backbone (described above) via T4 DNA ligation (NEB, M0202). .. The Sequencing:Article Title: Reprogramming of neuronal genome function and phenotype by astrocytes Article Snippet: The annealed duplex was diluted 1:200 and cloned into Esp3I-digested (NEB, R0734) and gel-purified (Qiagen, 28706) lentiviral backbone (described above) via T4 DNA ligation (NEB, M0202). .. The |